Label-Free Optofluidic Nanobiosensor Enables Real-Time Analysis of Single-Cell Cytokine Secretion

Celljet: Label-free Cell Printing via Real-time Impedance Flow Cytometry for Single Cell Analysis

The CellJet microfluidic dispenser chip prints single living cells encapsulated in free-flying droplets. Two sets of parallel facing electrodes in a 50 x 55 μm channel are applied to measure the presence and velocity of a single cell in real-time. Typically a 500 pl droplet is printed on demand, when a cell is at the nozzle. Feeding 20 μm polystyrene beads, a cell model, resulted in a peak-to-p...

A label free electrochemical nanobiosensor study

Single Cell Analysis of the Proinflammatory Responses of Mast Cell by a Real Time Secretion Assay

A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory respon...

Real-time single-cell imaging of protein secretion

Protein secretion, a key intercellular event for transducing cellular signals, is thought to be strictly regulated. However, secretion dynamics at the single-cell level have not yet been clarified because intercellular heterogeneity results in an averaging response from the bulk cell population. To address this issue, we developed a novel assay platform for real-time imaging of protein secretio...

A single-layer, planar, optofluidic Mach-Zehnder interferometer for label-free detection.

We have developed a planar, optofluidic Mach-Zehnder interferometer for the label-free detection of liquid samples. In contrast to most on-chip interferometers which require complex fabrication, our design was realized via a simple, single-layer soft lithography fabrication process. In addition, a single-wavelength laser source and a silicon photodetector were the only optical equipment used fo...